Chemi-Verse™ TNK1 Kinase Assay Kit

Catalog #

82159

$535 *

Size: 96 reactions

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Chemi-Verse™ TNK1 Kinase Assay Kit is designed to measure TNK1 (tyrosine kinase non receptor 1) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant TNK1 kinase (amino acids 106-390), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

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Synonyms

CD38 Negative Kinase 1, Tyrosine Kinase Non Receptor 1, Mitogen-Activated Protein Kinase 1, MAPK1, CD36 Molecule, CD36, RAF1, KOS1

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Product Data Gallery

Inhibition of TNK1 kinase activity by Staurosporine.

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Materials Required But Not Supplied

ADP-Glo™ Kinase Assay (Promega #V6930)
DTT (Dithiothreitol), 1M, optional
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog #	Name	Amount	Storage
80019	TNK1, GST-Th-Tag*	10 µg	-80°C
79334	5x Kinase Buffer 1	1.5 ml	-20°C
79686	500 µM ATP	50 µl	-20°C
	CSKtide (1 mg/ml)	500 µl	-20°C
79696	White 96-well plate	1	Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube.

UniProt #

Q13470

Background

TNK1, also known as tyrosine kinase non receptor 1 or thirty-eight-negative kinase -1, is part of the NRTK family (non-receptor tyrosine kinases), in particular the ACK sub-family. This sub-family is composed of two members, TNK1 and TNK2, and they are unique in that they have a putative ubiquitin association domain (UBA). TNK1 has a tissue-restricted expression profile in adults, being present in prostate, testis, ovaries, colon, and the small intestine. During development it is more widely expressed, and it is involved in hematopoiesis. TNK1 is involved in the primary lymphoid malignancies by mediating cell survival, and it has been shown to sensitize cancer cells to stress by reducing phosphorylation of STAT3 (signal transducer and activator of transcription 3) and STAT5 in L540 cells. TNK1 is inhibited by interacting with the protein 14-3-3, a binding that involves MARK (MAP/microtubule affinity-regulating kinase)-mediated phosphorylation. Regulation of its activity also involves ubiquitin. When active, TNK1 can induce growth-factor independent lymphoid cell proliferation in vitro. The use of an inhibitor, TP-5801, has shown efficacy in suppressing tumor growth in vitro and in vivo. The deeper understanding of the mode of action of this unique kinase and of the effects of its inhibition may open new therapeutic avenues.

References

Chan T., et al., 2021 Nature Communications 12: 5337.

Storage/Stability

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Shipping Temperature

-80°C

Notes

Troubleshooting Guide
Visit bpsbioscience.com/assay-kits-faq for detailed troubleshooting instructions. For all further questions, please email [email protected]

Contraindications

The final concentration of DMSO in the assay should not exceed 1%.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Kinases

Regulatory Status

Research Use Only

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Chemi-Verse™ TNK1 Kinase Assay Kit

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