CBL-B (Y363E), GST-Tag Recombinant

Catalog #

101407

$350 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Recombinant human CBLB (Cbl proto-oncogene B), encompassing amino acids 39-426. This construct contains an N-terminal GST-tag followed by a PreScission Protease Cleavage site to facilitate removal of the GST portion and contains the mutation of interest (Y363E). The recombinant protein was affinity purified.

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Synonyms

E3 ubiquitin-protein ligase CBL-B, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56, RING-type E3 ubiquitin transferase CBL-B, SH3-binding protein CBL-B, Signal transduction protein CBLB, RNF56, Nbla00127

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CBL-B Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

CBL-B (Y363E) (GST-39-426)

Mutation

Y393E

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.2 mM TCEP, and variable glutathione

72 kDa

Amino Acids

39-426

Genbank #

NM_170662

UniProt #

Q13191

Tag(s)

N-terminal GST-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to the protocol in CBL-B TR-FRET Assay Kit (BPS Bioscience #79575).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin E3 ligase

Regulatory Status

Research Use Only

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Datasheets/Protocols

101407 Lot 220412

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CBL-B (Y363E), GST-Tag Recombinant

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