CBL-B, GST-Tag (Human) Recombinant

Catalog #

80415

$360 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B), also known as E3 ubiquitin-protein ligase CBL-B, and RING finger protein 56, GenBank Accession No. NM_170662, a.a. 39 – 426 with N-terminal GST-Tag expressed in an E. coli expression system. MW=72 kDa.

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Synonyms

E3 ubiquitin-protein ligase CBL-B, RING finger protein 56, CBL, CBLB

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

CBL-B inhibition curve using a TR-FRET assay (#79575). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 0.2 mM TECP, 20% glycerol, various glutathione

72 kDa

Amino Acids

39 – 426

Genbank #

NM_170662

UniProt #

Q13191

Tag(s)

N-terminal GST-Tag

Background

CBL-B, a RING finger E3-ubiquitin ligase, acts as an intracellular checkpoint and a regulator of cytotoxic lymphocytes and NK cells. CBL-B binds E2-ubiqutin as well as target proteins such as TAM receptor TYRO3, forcing further degradation of TYRO3. CBL-B modulates innate immune responses and plays a key role in host defense to pathogens and anti-tumor immunity. Targeting CBL-B represents a potential therapeutic strategy for the management of human immune-related disorders such as autoimmune diseases, infections, tumors, and allergic airway inflammation. CBL-B has emerged as a new target of interest in the field of immunotherapy due to its ability to suppress cancer immune-evasion. CBL-B initiates anti-cancer immune responses in NK cells in both cytotoxic and effector T cells, promoting T-cell and NK cell activation.

References

1. Jeon, M.S., et al., Immunity. 2004; 21(2): 167-177.

2. Chiang, Y.J. et al., Nature. 2000; 403: 216-220.

Storage/Stability

At least 6 months at –80˚C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Assay Conditions

10 uL of reaction mixture contains 50 mM Tris, pH 7.4, 5 mM MgCl2, 0.01% Tween, 1 mM DTT, 0.3 µM Ub[Biotin], 100 nM Ube1, 1 mM ATP, 2 µM of UBCH5b, and various amount of CBL-B. The reaction was initiated with the addition of ATP, and incubated at 30° C for 3 hours. Tb labeled anti-GST antibody donor and XL665 labeled acceptor was added and incubated at room temperature for an hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitin E3 ligase

Regulatory Status

Research Use Only

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Datasheets/Protocols

80415_Lot160308-1.pdf

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

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CBL-B, GST-Tag (Human) Recombinant

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