Bald Lentiviral Pseudovirion (Luc-eGFP Dual Reporter)
The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains a firefly luciferase and eGFP cassette (Luc-P2A-eGFP) as the reporters, driven by a CMV promoter (Figure 1). The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.
Figure 1. Schematic of the Luciferase-P2A-eGFP Reporter in Bald Lentiviral Pseudovirion
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• HEK293 growth medium or use Thaw Medium 1 (BPS Bioscience #60187): MEM with 10% FBS, 0.1 mM nonessential amino acids, 1 mM sodium pyruvate, 1% Penicillin/Streptomycin (Hyclone #SV30010.01).
• ACE2-HEK293 Recombinant Cell Line (BPS Bioscience, #79951)
• SARS-CoV-2 Spike Pseudotyped Lentivirus (Luc-eGFP dual reporter) (BPS Bioscience, #79982)
• 96-well tissue culture treated, white clear-bottom assay plate (Corning, #3610)
• ONE-Step™ luciferase assay system (BPS Bioscience, #60690)
Since the virus is lacking the envelope glycoproteins and cannot transduce target cells, functional titer of this product cannot be determined. Based on p24 values, the approximate number of lentiviral particles (LP) of this product is ~109 LP/ml.
The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
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