Bald Lentiviral Pseudovirion (eGFP Reporter)

Catalog #

79987

$835 *

Size: 500 µl x 2

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

The bald lentiviral pseudovirion was produced without envelope glycoproteins such as VSV-G or SARS-CoV-2 spike. It contains the eGFP gene driven by a CMV promoter (Figure 1) as the reporter. The bald lentiviral pseudovirion can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.

Figure 1. Schematic of the eGFP Reporter in Bald Lentiviral Pseudovirion (eGFP Reporter)

Figure 1. Schematic of the eGFP Reporter in Bald Lentiviral Pseudovirion (eGFP Reporter)

This product has been cited 8 times.

●

Synonyms

Severe acute respiratory Coronavirus Spike 2, pseudovirus, lentivirus

●

Product Data Gallery

Product Info

Storage and Usage

Citations8

Supplied As

Based on p24 values, the approximate number of lentiviral particles (LP) of this product is ~ 10⁹LP/ml.

Materials Required But Not Supplied

• HEK293 growth medium or use Thaw Medium 1 (BPS Bioscience #60187): MEM supplemented with 10% FBS, 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).
• ACE2-HEK293 Recombinant Cell Line (BPS Bioscience, #79951)
• SARS-CoV-2 Spike Pseudotyped Lentivirus (eGFP Reporter) (BPS Bioscience, #79981)

Formulation

The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Instructions for Use

See assay protocol for detailed instructions.

Shipping Temperature

-80°C

Notes

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Bald Lentiviral Pseudovirion (eGFP Reporter)

PRODUCTS

SERVICES

RESOURCES

COMPANY