B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

Catalog #

78363

$11,095 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Beta-2-microglobulin, B2M, CDABP0092, HDCMA22P

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Product Data Gallery

Genomic Sequencing of B2M in the B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

B2M Expression in B2M knockout NFAT Luciferase Reporter Jurkat cells

Stimulation of NFAT Luciferase activity with anti-CD3 Agonist Antibody

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat (clone E6-1), human T lymphoblast, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2R	BPS Bioscience #78411

Materials Required for Cellular Assay

Name	Ordering Information
Anti-CD3 Agonist Antibody	BPS Bioscience #71274
NFAT Luciferase Jurkat Recombinant Cell Line	BPS Bioscience #60621
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well tissue culture-treated white, clear-bottom plate
Luminometer

UniProt #

P61769

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Beta-2-Microglobulin is a required component of Major Histocompatibility Complex (MHC) class 1 molecules, which present peptide fragments from within the cell to cytotoxic T cells as part of the adaptive immune system. The protein forms amyloid fibrils in some pathological conditions. A mutation in this gene drives hypercatabolic hypoproteinemia. B2M plays an essential role both in governing MHC class I molecule stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

NFAT (Nuclear factor of activated T-cells) is a family of transcription factors that has an important function in immune responses, for example by inducing the expression of various cytokines (such as IL-2-3-4 and TNF-alpha) in T cells. NFAT is regulated by Ca2+ and the Ca2+/calmodulin-dependent serine phosphatase, calcineurin. NFAT is also activated by the T cell Receptor (TCR) in T cells.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 2R (BPS Bioscience #78411): PMI 1640 medium supplemented with 10% FBS, plus 400 µg /ml of Hygromycin B

Media Required for Functional Cellular Assay

Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Applications

Study the consequences of B2M knock down or use as control in immune-oncology product development

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line

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