B2M Knockout iPS Cell Line

Catalog #

82161

$7,350 *

Size: 1 vial

Qty

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The B2M Knockout iPS Cell Line is an iPS cell line that has been edited using CRISPR/Cas9 technology to delete B2M (Beta-2-Microglobulin) gene expression. The B2M-targeting CRISPR/Cas9 editing agents were delivered via transduction with B2M (Human) CRISPR/Cas9 Lentivirus (Non-Integrating) (BPS Bioscience #78341).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

iPSC

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Product Data Gallery

Flow cytometry analysis of OCT expression in B2M Knockout iPS Cell Line.

Flow cytometry analysis of B2M expression B2M Knockout iPS Cell Line.

Genome sequencing of B2M in the B2M Knockout iPS Cell Line.

Flow cytometry analysis of HLA expression in B2M Knockout iPS Cell Line.

Product Info

Storage and Usage

Citations

Host Cell Line

Non-Disease Human iPS Cell Line (iXCells 30HU-002)

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of iPS Cell Freezing Medium

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
mTeSR™ Plus	Stem Cell Technologies #100-0276
Matrigel™	Corning #354230
DMEM/F12	Thermo Fisher #11330032
Thiazovivin	BPS Bioscience #78506
RelesR	Stem Cell Technologies #05872
Accutase	Thermo Fisher #A1110501

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The discovery by Yamanaka and colleagues in 2007 that 4 factors were sufficient to reprogram terminally differentiated fibroblasts into pluripotent stem cells launched the advent of human induced pluripotent stem (iPS) cell technology. These human iPS cells are capable of both self-renewal and differentiation down all three germline lineages and provide both a tool to model human development and disease in the relevant differentiated human cell types, and a unique opportunity for high throughput drug screening and cell therapy development.

B2M (Beta-2-Microglobulin) forms a heteromeric complex with HLA-A, HLA-B and HLA-C molecules to generate the functional Class I MHC molecule responsible for antigen presentation to T-Cells. Class I MHC molecules are present on the surface of all nucleated cells and play a role in the rejection of organs or allogenic cells during organ transplantation and cell therapy. B2M is an attractive target to reduce the immunogenicity of iPS cell-derived allogenic cell therapies.

References

Cong L., et al., 2013 Science. 339(6121):819-23.
Gerace D., et al., 2023 Cell Reports Medicine 4: 100879
Jinek M., et al., 2012 Science. 337(6096):816-21.
Mali P., et al., 2013 Science. 339(6121):823-6.
Lanza R., et al., 2019 Nat Rev Immunol 19: 723-733.
Takahashi K., et al., 2007 Cell. 131(5):861–872.
Yang L., et al., 2013 Nucleic Acids Res. 41(19):9049-61

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media formulations by BPS Bioscience is highly recommended. Other formulations of media may result in suboptimal performance.

Media Required for Cell Culture
iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin.

Complete iPSC Thaw Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin, and 1 µM Thiazovivin.

Thiazovivin is unstable once diluted in the medium. Prepare just enough to proceed with thawing of the cells

iPSC Growth Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin.

PSC Passage Medium: mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin, and 1 µM Thiazovivin.

2X IPS Cell Freezing Medium: 80% mTeSR™ Plus supplemented with 1% Penicillin/Streptomycin, 1 µM Thiazovivin, and 20% DMSO (vol/vol).

Applications

Assess the role of B2M knockout in iPS cell biology and differentiation.
Assess the impact of B2M knockout in cell therapy research and development.

Shipping Temperature

-80°C

Notes

License Disclosure
The iPSC technology is protected by several patents, including US patent Nos. 8048999, 8058065, 8129187, 8278104, 8530238, 8900871, 9404124, 9499797, 10519425, and patent pending, for which iPS Academia Japan, Inc. has been granted license rights with a sub-licensable right.

The purchase of this cell line grants you a 10-year license to use this cell line in your immediate laboratory for internal research purposes. Commercial use of this cell line is not allowed. Commercial use requires the appropriate license from iPS Academia Japan, Inc.

This cell line is for research use only, not for therapeutic or prophylactic use in humans or animals. Use in humans is strictly prohibited.

This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of the cell line in any way. Modification of this cell line or transfer to another facility of the cells requires a separate license or additional fees; contact [email protected] for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use and does not accept any liability in connection with the handling or use of the cell line.

Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Gene & Cell Therapy

Regulatory Status

Research Use Only

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B2M Knockout iPS Cell Line

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