B2M/CIITA Double Knockout THP-1 Cell Line

Catalog #

78391

$9,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Both B2M (Beta-2-Microglobulin) and CIITA (Class II Transactivator) have been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

Genomic Sequencing of B2M in the B2M/CIITA Double Knockout THP-1 Cell Line

B2M Expression in B2M/CIITA Double Knockout THP-1 cells

Genomic Sequencing of CIITA in the B2M and CIITA Double Knockout THP-1 Cell Line

CIITA Expression in B2M/CIITA Double Knockout THP-1 cells

Product Info

Storage and Usage

Citations

Host Cell Line

THP-1, human monocyte, suspension, derived from an acute monocytic leukemia patient.

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184

UniProt #

P61769, P33076

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Beta-2-Microglobulin is a required component of Major Histocompatibility Complex (MHC) class I molecules, which present peptide fragments from within a cell to cytotoxic T cells as part of the adaptive immune system. B2M plays an essential role both in governing MHC class I molecule stability and in promoting antigen binding and presenting the antigen to CD3/TCR complex of CD8+ T cells.

CIITA (Class II Transactivator, also known as Class II Major Histocompatibility Complex Transactivator) acts as a coactivator for MHC class II-specific gene expression. IFN-Ƴ induces CIITA gene expression via the JAK1 and STAT1 pathways. Defects in CIITA have been implicated in Bare Lymphocyte Syndrome (BLS), which is characterized by the absence of MHC class II transcription and severe immunodeficiencies.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 2 (BPS Bioscience, #60184): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Applications

Study the consequences of deficient MHC class I and II.
Study T cell activation, antigen presentation, and immune responses.
Useful for the development of improved universal CAR-T or other effector cells.

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and
8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR-T

Regulatory Status

Research Use Only

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B2M/CIITA Double Knockout THP-1 Cell Line

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