Abl Kinase, His-tag Recombinant
Catalog #
40411
$375
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Description
Recombinant human protein kinase ABL1 (variant b) encompassing amino acids 248-531 that correspond to the kinase domain. In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308). The protein was affinity purified.
This product has been cited 1 time.
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Synonyms
ABL, ABL1, ABL-1, Abelson murine leukemia viral oncogene homolog 1, JTK7, Proto-oncogene c-Abl, p150, c-ABL, v-abl, CHDSKM
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Product Data Gallery
Product Info
Storage and Usage
Citations1
Species
Human
Construct
Abl Kinase (His-248-531)
Host Species/Expression System
Sf9 insect cells
Purity
≥90%
Format
Aqueous buffer solution
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 130 mM Imidazole, 20% glycerol
MW
35 kDa
Amino Acids
248–531
Specific Activity
2,700 pmol/min/μg
Genbank #
NM_007313
UniProt #
P00519
References
1. Cao, C., et al. 2003. J. Biol. Chem. 278(15):12961–7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3):356–364.