JARID1A (KDM5A) Chemiluminescent Assay Kit

Catalog #

50513

$715 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The JARID1A Chemiluminescent Assay Kit is designed to measure JARID1A activity for screening and profiling applications. The key to the JARID1A Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, JARID1A enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

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Synonyms

KDM5A, RBBP2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

Kit comes in a convenient format, with a 96-well strip plate precoated with methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JARID1A for 100 enzyme reactions.

Materials Required But Not Supplied

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Format

Catalog #	Component	Amount	Storage
50110	JARID1A (KDM5A, RBBP2)	10 µg	-80°C	Avoid freeze/ thaw cycles!
52140M	Primary antibody 13	25 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	10 µl	-80°C
	4x JARID1A Direct Assay Buffer	3 x 1 ml	-80°C
52100	Blocking buffer 4	50 ml	+4°C
79670	ELISA ECL chemiluminescent substrate A (transparent bottle)	6 ml	+4°C
79670	ELISA ECL chemiluminescent substrate B (brown bottle)	6 ml	+4°C
	8-well strip plate module precoated with histone substrate	1	+4°C

UniProt #

P29375

Background

JARID1A is a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation.

References

Klose, R.J., et al. Cell 2007; 128: 889.

Storage/Stability

6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for studying enzyme kinetics and HTS applications.

Shipping Temperature

-80°C (dry ice)

Notes

The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request.

Contraindications

DMSO >1%, strong acids or bases, ionic detergents, high salt

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Demethylase

Regulatory Status

Research Use Only