VRK1, GST-tag Recombinant

Catalog #

40397

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human vaccinia related kinase 1 (VRK1), GenBank Accession No. NM_003384, full length with N-terminal GST tag, MW=71 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

human

Host Species/Expression System

baculovirus

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, and 0.25 mM DTT.

71 kDa

Amino Acids

full length

Specific Activity

≥ 2 pmol/min/µg

Genbank #

NM_003384

UniProt #

Q99986

Tag(s)

N-terminal GST-tag

References

1. Nezu, J., et al., Genomics 1997;45:327-331.
2. Renbaum, P., et al., Am. J. Hum. Genet. 2009;85:281-289.

Storage/Stability

Store at -70°C. Stable for 12 months when stored as directed.

Assay Conditions

5 mM MOPS, 2.5 mM beta-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate VRK1 with 0.25 mg/ml dephosphorylated casein as substrate and [33P]-ATP at 30&Deg;C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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VRK1, GST-tag Recombinant

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