ULK2 Kinase Assay Kit
The ULK2 Kinase Assay Kit is designed to measure ULK2 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.
- Kinase-Glo MAX (Promega #V6071)
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40294 | ULK2* | 5 µg | -80°C |
| 79334 | Kinase assay buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 100 µl | -20°C |
| 40535 | Myelin basic protein (MBP), 5 mg/ml | 200 µl | -20°C |
| 79696 | 96-well plate, white | 1 | Room Temp. |
*The concentration of the protein is lot-specific and will be indicated on the tube.
ULK2 (also known as Unc51.2, unc-51 like autophagy activating kinase 2) is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy in response to nutrient starvation. Mammalian ATG13 binds ULK2 and mediates the interaction of the ULK protein with FIP200. The binding of ATG13 stabilizes and activates ULK and facilitates the phosphorylation of FIP200 by ULK. The ULK-ATG13-FIP200 complex is a direct target of mTOR and an important regulator of autophagy in response to mTOR signaling (1). Yeast 2-hybrid assays showed that the C-terminus of ULK2 binds the C-terminus of SynGAP, a negative regulator of Ras that is associated with neural development (2). Alterations in ULK signaling pathways may be involved in the formation of autophagy-regulated Lewi bodies, which have been associated with Parkinson’s disease (3).
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