ULK1, FLAG-Tag Recombinant

Catalog #

40099

$405 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human ULK1, also known as ATG1, UNC51, and hATG1 , encompassing amino acids 2–1050 (end). This construct contains an N-terminal FLAG-tag. This protein was affinity purified.

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Synonyms

ATG1, UNC51, Unc51.1, FLJ38455, Unc-51-Like Kinase, Autophagy-Related Protein 1, Human ULK1, Ulk 1, ULK1 Protein

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

ULK1 Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

ULK1 (FLAG-2-1050(end))

Host Species/Expression System

HEK293

Purity

≥40%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, and variable FLAG peptide

114 kDa

Amino Acids

2-1050 (end)

Specific Activity

≥85 pmol/min/µg

Genbank #

NM_003565

UniProt #

O75385

Tag(s)

N-terminal FLAG-tag

Background

The ULK1 protein is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

References

1. Ganley I.G. et al., J. Biol. Chem. 284 (18), 12297-12305 (2009).
2. Chan E.Y. et al., Mol. Cell. Biol. 29 (1), 157-171 (2009).
3. AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1 (2013)
5. mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6 (2013)

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to ULK1 Kinase Assay Kit (BPS Bioscience #78362) with various amounts of ULK1.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40099 Lot 230320-1

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ULK1, FLAG-Tag Recombinant

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