TNFR2:TNF-alpha[Biotinylated] Inhibitor Screening Assay Kit
The TNFR2: TNFalpha[Biotinylated] Inhibitor Screening Assay Kit is designed to measure the binding of TNFR2 (tumor necrosis factor receptor 2) to TNF-alpha (TNFα) for screening and profiling applications. The TNFR2:TNF-alpha [Biotinylated] Inhibitor Screening Assay Kit comes in a convenient 96-well format, with enough recombinant purified biotinylated TNF-alpha (amino acids 29-216), TNFR2 (amino acids 23-257), blocking and assay buffer and detection reagents for 100 enzyme reactions.
Need us to run inhibitor screens or profile your compounds against TNFR2:TNF-alpha[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.
This product has been cited 4 times.
- 1x PBS (phosphate buffer saline) Buffer
- Luminometer or microplate reader capable of reading chemiluminescence
- Adjustable micropipettor and sterile tips
- Rotating or rocker platform
| Catalog # | Name | Amount | Storage |
| 79363 | TNFR2, Fc-Fusion (IgG1), His-Avi-Tag* | 10 µg | -80°C |
| TNF-alpha, Biotin-Labeled* | 2 µg | -80°C | |
| 79311 | 3x Immuno Buffer 1 | 50 ml | -20°C |
| 79728 | Blocking Buffer 2 | 50 ml | +4°C |
| 79742 | Streptavidin-HRP | 10 µl | +4°C |
| 79670 | ELISA ECL Substrate A (translucent bottle) | 6 ml | Room Temp |
| ELISA ECL Substrate B (brown bottle) | 6 ml | Room Temp | |
| 79699 | 96-well white microplate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
Tumor Necrosis Factor Receptor 2 (TNFR2, also known as TNFRSF1B or CD120b) is a transmembrane receptor of the TNF protein superfamily that binds the pleiotropic pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). TNFR2 can be found in several T cell subsets such as regulatory CD8+ T cells (Tregs) and CD4+ tumor-infiltrating T cells, myeloid lineage cells and some cancer types, and it is involved in autoimmune diseases, graft versus host disease and cancer. It has become an attractive target for cancer immunotherapy, where its different functions as oncogene and immune regulator are being explored. TNFR2 also exhibits neuroprotective properties and promotes tissue regeneration, making it a promising potential therapeutic target for the treatment of Alzheimer’s disease.
Vanamee ES., et al., 2017 Trends. Mol. Med. 23(11): 1037-1046
Chen X., et al., 2017 Sci. Signal. 10(462): eaal2328
Ortí-Casañ. N., et al., 2019 Front. Neurosci. 13: 49.
Medler J, et al., 2022 Cancers 14(11): 2603
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