The TNFR2 (Tumor Necrosis Factor Receptor 2, or TNR1B) Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with Human TNFR2 (NM_001066.3) driven by a CMV promoter. The lentiviruses also transduce a puromycin selection gene (Figure 1).
Figure 1. Schematic of the lenti-vector used to generate the TNFR2 Lentivirus.
●
Synonyms
NFRSF1B, TNFBR,Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, TNF-alpha receptor, CD120b
●
Product Data Gallery
Expression of human TNFR2 in HEK293 cells transduced with TNFR2 lentiviruses.
Two vials (500 µl x 2) of lentivirus at a titer ≥107 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Formulation
The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.
Background
Tumor Necrosis Factor Receptor 2 (TNFR2, also known as TNR1B, TNFRSF1B, or CD120b) is a transmembrane receptor of the TNF protein superfamily that binds the pleiotropic pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). TNFR2 can be found in several T-Cell subsets such as regulatory CD8+ T-Cells (Tregs) and CD4+ tumor infiltrating T cells, myeloid lineage cells and some cancer types, and it is involved in autoimmune diseases, graft versus host disease and cancer. It has become an attractive target for cancer immunotherapy, where its different functions as oncogene and immune regulator are being explored. TNFR2 also exhibits neuroprotective properties and promotes tissue regeneration, making it a promising potential therapeutic target for the treatment of Alzheimer’s disease.
Storage/Stability
Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.
Applications
Expression of human TNFR2 in cells of interest.
Generate stable cell lines expressing human TNFR2 (puromycin resistant).
Shipping Temperature
-80°C
Notes
To generate a TNFR2 stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of puromycin (as pre-determined from a killing curve), for antibiotic selection of transduced cells. Visit: https://bpsbioscience.com/cell-line-faq for guidelines on how to perform a kill curve.
Biosafety The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Powered by Bioz
