TMPRSS2 – Vero E6 Recombinant Cell Line

Catalog #

78081

$11,650 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant clonal stable Vero E6 cell line constitutively expressing full length human TMPRSS2 (Genbank #NM_005656.4).

This product has been cited 34 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

coronavirus, covid, covid-19, covid19, TMPRSS2 cell line, protease cell line, TMPRSS2 inhibition, stable TMPRSS2 cell line

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Product Data Gallery

Expression of TMPRSS2 in TMPRSS2 Vero E6 cell line

Enhanced infectivity of SARS-CoV-2 Spike pseudotyped lentivirus associated with TMPRSS2 expression in Vero E6 cells

Inhibition of SARS-CoV-2 Spike pseudovirus infection by protease inhibitors in ACE2 HEK293 cells and TMPRSS2 Vero E6 cells

Inhibition of SARS-CoV-2 Spike pseudovirus infection by Camostat mesylate in TMPRSS2 Vero E6 cells

Neutralization of SARS-CoV-2 Spike speudovirus infection by anti-SARS-CoV-2 Spike antibody

Inhibition of TMPRSS2 proteolytic activity by Camostat mesylate in TMPRSS2 Vero E6 cells

Inhibition of TMPRSS2 proteolytic activity by Gabexate mesylate in TMPRSS2 Vero E6 cells

Product Info

Storage and Usage

Citations34

Host Cell Line

Vero C1008 cells, Vero 76 clone, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Media Required for Cell Culture

Name	Ordering Information
Thaw Medium 1	BPS Bioscience #60187
Growth Medium 1P	BPS Bioscience #78095

Materials Used in Cellular Assays

Name	Ordering Information
H-CELL-100 Medium	Wisent Bio Products #001-035-CL
Spike (SARS-CoV-2) Pseudotyped Lentivirus (Luc Reporter)	BPS Bioscience #79942
E-64d	Sigma #E8640
Camostat mesylate	Sigma #SML0057
Gabexate mesylate	Sigma #SML2964
Boc-QAR-AMC Fluorogenic Peptide Substrate	R&D Systems #ES014
Vero E6 cell line	ATCC #CRL-1586
ACE2 – HEK293 cell line	BPS Bioscience #79951
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
Luminometer
Fluorescence plate reader

Genbank #

NM_005656.4

UniProt #

O15393

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Human transmembrane serine protease 2 (TMPRSS2) is an enzyme primarily expressed by endothelial cells across the respiratory and digestive tracts. It is involved in viral entry and spread of coronaviruses including SARS-CoV-2, the virus that causes COVID-19. Blocking TMPRSS2 could potentially be an effective clinical therapy for COVID-19.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin

Growth Medium 1P (BPS Bioscience #78095): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, 1% Penicillin/Streptomycin and 3 μg/ml of Puromycin

Media Required for Functional Cellular Assay

Thaw Medium 1 (BPS Bioscience #60187): MEM medium supplemented with 10% FBS, 1% non-essential amino acids, 1 mM Na pyruvate, and 1% Penicillin/Streptomycin.

Assay Medium: H-CELL-100 Medium (Wisent Bio Products, #001-035-CL)

Applications

Screen human TMPRSS2 inhibitors

Shipping Temperature

-80°C

Notes

This cell line may require a CITES certificate for export, which can incur additional fees. Please contact BPS Bioscience at international@bpsbioscience.com or your local distributor for more information.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Coronavirus

Regulatory Status

Research Use Only

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TMPRSS2 – Vero E6 Recombinant Cell Line

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