The transforming growth factor beta receptor (TGFβR1, also known as ALK5) Kinase Assay Kit is designed to measure the serine/threonine activity of TGFβR1 (ALK5) for screening and profiling applications using ADP-Glo® as a detection reagent.
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Synonyms
TGFBR1, ALK5, SKR4, TGF-beta receptor type-1, TGFR-1, Activin A receptor type II-like protein kinase of 53kD, Activin receptor-like kinase 5 (ALK-5; ALK5), Serine/threonine-protein kinase receptor R4 (SKR4), TGF-beta type I receptor
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Product Data Gallery
Inhibition of TGFβR1 (ALK5) kinase Activity by A 83-01 (Medchemexpress #HY-10432)
The assay kit comes in a convenient 96-well format, with enough purified recombinant TGFßR1 (ALK5) kinase, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
*The concentration of the protein is lot-specific and will be indicated on the tube
UniProt #
P36897
Background
The transforming growth factor beta (TGFB) signaling pathway regulates a wide range of biological and cellular processes including cell growth, differentiation, survival/apoptosis, and migration. TGFβ binds to a type II receptor dimer, which recruits a type I receptor dimer forming a hetero-tetrameric complex. The TGFβ receptor is a transmembrane serine/threonine kinase that phosphorylates proteins of the SMAD (Mothers against decapentaplegic homolog) family. Due to its role in immunosuppression, cancer, and inflammatory diseases, among others, this receptor is an attractive therapeutic target.
Storage/Stability
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Applications
Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput (HTS) applications.
The final concentration of DMSO in the assay should not exceed 1%.
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.
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