PRAME TCR CD8+ NFAT-Luciferase Reporter Jurkat Cell Line

Catalog #

78997

$14,500 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

PRAME TCR CD8⁺ NFAT-Luciferase Reporter Jurkat Cell Line was generated from T Cell Receptor (TCR) Knockout NFAT Luciferase Reporter Jurkat Cell Line (BPS Bioscience #78556) by overexpression of human CD8 (NM_001768.6) and a PRAME (Preferentially Expressed Antigen in Melanoma)-directed TCR using lentiviral transduction (CD8a Lentivirus #78648 and PRAME-Specific TCR Lentivirus #78959). This PRAME TCR specifically recognizes an antigen PRAME peptide, amino acids peptide 425-433 (SLLQHLIGL).

Figure 1: Illustration of the functional co-culture assay used to validate the PRAME TCR CD8⁺ NFAT-Luciferase Reporter Jurkat Cell Line.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, Preferentially expressed antigen of melanoma

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Product Data Gallery

Flow cytometry analysis of the expression of PRAME TCR in PRAME TCR CD8+ NFAT-Luciferase Reporter Jurkat Cell Line.

PRAME TCR CD8+ NFAT-Luciferase Reporter Jurkat Cell Line activation using T2 cells as APC.

Product Info

Storage and Usage

Citations

Host Cell Line

Jurkat (clone E6-1), human T lymphoblast, suspension

Species

Human

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 2	BPS Bioscience #60184
Growth Medium 2T	BPS Bioscience #78756
Assay Medium 2D	BPS Bioscience #78755
CD8⁺ TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line	BPS Bioscience #78757
T2 Cell Line	ATCC #CRL-1992
PRAME Peptide (425-433)	BPS Bioscience #78991
PE anti-human α/β T Cell Receptor Antibody	BioLegend #306707
ONE-Step™ Luciferase Assay System	BPS Bioscience #60690
96-well tissue culture plate, white, clear bottom

UniProt #

P78395

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

PRAME (Preferentially Expressed Antigen in Melanoma) is a protein with a profile of expression in normal tissues highly restricted to testis, ovarium, and endometrium. However, it is found at high levels in several cancer types, such as melanoma, breast, and lung cancer. It is also found in cells of patients with AML (acute myeloid leukemia) and Hodgkin’s lymphoma. Overexpression seems to block retinoic acid (RA)-mediated cell proliferation, differentiation, and apoptosis, contributing to tumorigenesis. Its expression pattern makes it an attractive target for immunotherapy. It is a membrane-bound protein, and it is thus a good target for TCR (T cell receptor)-T cells and anti-PRAME vaccines. Several clinical trials are ongoing and have demonstrated the clinical potential of targeting PRAME in melanoma, lung cancer and other solid tumors. Further studies into the functions of this protein will bring new clinical advances in cancer therapy.

CD8 (Cluster of Differentiation 8) is a co-receptor of TCR and a typical marker of cytotoxic T cells. The TCR protein complex is found on the surface of T cells and is responsible for recognizing antigens bound to MHC (Major Histocompatibility Complex) molecules. Stimulation of the TCR results in activation of downstream NFAT (Nuclear factor of Activated T-cells) transcription factors that induce the expression of various cytokines such as interleukin-2 to 4, and TNF-alpha. The use of engineered TCR allows T cells to target specific antigens present in cancer cells via the MHC, expanding the portfolio of antigens that can be targeted in cancer cell therapy.

References

Xu Y., et al., 2020 Cell Prolif. 53(3):e12770.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Growth Media

For best results, the use of validated and optimized media by BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 2 (BPS Bioscience #60184): RPMI 1640 medium supplemented with 10% FBS, and 1% Penicillin/Streptomycin.

Growth Medium 2T (BPS Bioscience #78756): RPMI 1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 0.5 mg/ml of Geneticin, 100 µg/ml Hygromycin B, and 0.25 µg/ml puromycin.

Media Used in Functional Cellular Assay
Assay Medium 2D (BPS Bioscience #78755): RPMI 1640 medium supplemented with 1% FBS.

Applications

Design and optimize co-culture bioassays for PRAME-specific TCR cell evaluation.
Use as a positive control in experiments evaluating PRAME TCR cells.
Use as an in vitro system to measure vaccine T cell immunogenicity.

Shipping Temperature

-80°C

Notes

The CRISPR/CAS9 technology is covered under numerous patents, including U.S. Patent Nos. 8,697,359 and 8,771,945, as well as corresponding foreign patents applications, and patent rights.

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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PRAME TCR CD8+ NFAT-Luciferase Reporter Jurkat Cell Line

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