MDM2 TR-FRET Assay Kit

Catalog #

79773

$1,070 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Human Mouse double minute 2 homolog (MDM2) is an E3 ubiquitin-protein ligase that functions as a negative regulator of T-cell activation. It is a potential drug target in cancer immunotherapy. It utilizes biotin-labeled ubiquitin and a terbium-labeled anti-GST antibody to complete the TR-FRET pairing. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications.

Need us to run inhibitor screens or profile your compounds against MDM2? Check out our Ubiquitination Screening Services or DNA Replication & Repair Screening Services.

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Synonyms

Mdm2,mouse double minute 2, human homolog, Hdm2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

TR-FRET

Supplied As

The MDM2 TR-FRET Assay Kit is designed to measure MDM2 auto-ubiquitination activity in a homogeneous 384 reaction format.

Materials Required But Not Supplied

Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format

Catalog Number	Component	Amount	Storage
80301	UBE1 (E1)	25 µg	-80°C	*Avoid freeze/ thaw cycles!*
80314	UBCH5b (E2)	200 µg	-80°C
80751	Human MDM2 (E3), GST-tag	10 µg	-80°C
	Biotin-Ubiquitin	400 µl	-80°C
	ATP (400 µM)	150 µl	-80°C
	CBL Assay Buffer	2 x 10 ml	-80°C
	Tb-labeled donor	10 µl	-20°C
	Dye-labeled acceptor	10 µl	-20°C
	White, nonbinding Corning, low volume microtiter plate	1	Room Temp.

UniProt #

UBE1: P22314; UBCH5b: P62837; MDM2: Q00987

References

1. Wienken, M. et al., J. Mol. Cell Biol. 2017; 9(1): 74-80.
2. Shaikh, M.F. et al., Ann. Clin. Lab. Sci. 2016; 46(6): 627-34.

Storage/Stability

At least 6 months from date of receipt when stored as directed.

Instructions for Use

See assay protocol for detailed instructions.

Applications

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Ubiquitin

Regulatory Status

Research Use Only