MDM2, GST-tag Recombinant

Catalog #

100409

$375 *

Size: 20 µg

Qty

Bulk/Custom

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Description

Recombinant human MDM2 (Mouse double minute 2 homolog), full length encompassing amino acids 2-498 (end). In this construct the N-terminal end of MDM2 is fused with GST. A short peptide linker placed between GST and the protein includes a PreScission Protease Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

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Synonyms

Mdm2, mouse double minute 2, human homolog, Hdm2, Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, HDMX, LSKB, hdm2, ACTFS, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2

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Product Data Gallery

4-20% SDS-Page Coomassie Staining

MDM2 Activity

MDM2 inhibition curve using a TR-FRET assay (#78302). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

MDM2 (GST-Pre-2-498(end))

Host Species/Expression System

E. coli

Purity

52%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT

83 kDa

Amino Acids

2-498(end)

Genbank #

NM_002392

UniProt #

Q00987

Tag(s)

N-terminal GST-tag

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was performed according to MDM2 TR-FRET Assay Kit (BPS Bioscience #79773) with MDM2 titrated from 157.5 ng- 0 ng.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Ubiquitination

Regulatory Status

Research Use Only

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Datasheets/Protocols

100409 Lot 210514

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MDM2, GST-tag Recombinant

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