Insulin receptor (INSR), GST-tag Recombinant

Catalog #

40241

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human insulin receptor (INSR), GenBank Accession No. NM_001079817, a.a. 999-end with an N-terminal GST-tag, expressed in a baculovirus expression system. MW=67 kDa.

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Synonyms

INSR, insulin receptor

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥57%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 16 mM Glutathione, 3 mM DTT and 20% glycerol

67 kDa

Amino Acids

999–end

Specific Activity

752 pmol/min/μg

Genbank #

NM_001079817

UniProt #

P06213

Tag(s)

N-terminal GST-tag

References

1. Zhou, P. et al. J. Pediatr. Endocrinol. Metab. 21 (4), 369-375 (2008).
2. Machorro-Lazo, M.V. et al., Gynecol. Obstet. Invest. 67 (1), 14-19 (2008).

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

40 mM Tris-HCL pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.1 mg/ml Alextide substrate and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, autophosphorylation and phosphorylation of target substrates, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Receptor Tyrosine

Regulatory Status

Research Use Only