Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of a chimeric receptor consisting human ICOS (also known as inducible T-cell costimulator or CD278, Genbank Accession #NM_012092) and the cytoplasmic domain of human CD3 zeta.
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Synonyms
T-cell costimulator, CD278
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Product Data Gallery
The ICOS/NFAT Reporter Activities Stimulated by ICOSL-CHO Cells
Dose response of anti-ICOS agonist antibody on ICOS/NFAT Reporter-Jurkat cells
FACS Analysis of Cell Surface Expression of ICOS in ICOS/NFAT Reporter-Jurkat cells
Each vial contains 2 x 106 cells in 1 ml of 10% DMSO in FBS.
UniProt #
Q9Y6W8
Mycoplasma Testing
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination.
Background
ICOS is a costimulatory molecule of the CD28 cell surface receptor superfamily that is expressed on activated T-cells. ICOS is involved in T-cell responses upon binding with its ligand, ICOSL (also known as B7-H2, CD275), which is normally expressed on B-cells, dendritic cells and monocytes. ICOS expression confers an activated phenotype and a strong suppressive capacity to intra-tumoral regulatory T-cells. The ICOS/ICOSL pathway is a key target for cancer immunotherapy.
Storage/Stability
Store in liquid nitrogen immediately upon receipt.
Growth Media
For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.
Growth Medium 2F (BPS Bioscience #79669): RPMI1640 medium supplemented with 10% FBS, 1% Penicillin/Streptomycin, 1 mg/ml of Geneticin, and 0.5 μg/ml of Puromycin
Instructions for Use
See data sheet for detailed culturing and assay protocol.
Shipping Temperature
-80°C
Notes
License Disclosure Visit bpsbioscience.com/license for the label license and other key information about this product.