ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit

Catalog #

79673

$725 *

Size: 96 Reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of ICOS:ICOSL signaling. The key to this kit is the high sensitivity of detection of biotin-labeled ICOS by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, ICOSL is coated on a 96-well plate. Next, ICOS is incubated with ICOSL on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

Need us to run inhibitor screens or profile your compounds against ICOSL:ICOS[Biotinylated]? Check out our Immunotherapy Biochemical Screening Services.

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Synonyms

B7 homolog 2, CD275, ICOSL, ICOSLG, B7-related protein (B7RP), B7-H2

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Chemiluminescent

Supplied As

This kit comes in a convenient 96- well format, with biotin-labeled ICOS, purified ICOSL, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

Materials Required But Not Supplied

PBS (Phosphate buffered saline)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips

Format

Catalog #	Component	Amount	Storage
71130	B7-H2 (CD275, ICOSL), Fc fusion (Human) HiP™	10 µg	-80°C	Avoid freeze/ thaw cycles!
11257	ICOS (CD278), Biotin	5 µg	-80°C
79311	3x Immuno Buffer 1	50 ml	-20°C
79728	Blocking Buffer 2	50 ml	+4 °C
79742	Streptavidin-HRP	10 µl	+4°C
79670	ELISA ECL substrate A (transparent bottle)	6 ml	Room Temp
79670	ELISA ECL substrate B (transparent bottle)	6 ml	Room Temp
79699	96-well white microplate	1	+4°C

UniProt #

ICOSL: O75144; ICOS: Q9Y6W8

Background

ICOS (Inducible T-cell Costimulator, CD278) is a costimulatory molecule of the CD28 cell surface receptor superfamily that is expressed on activated T-cells. ICOS is involved in T-cell responses upon binding with its ligand, ICOSL (also known as B7-H2, CD275), which is normally expressed on B-cells, dendritic cells and monocytes. ICOS expression confers an activated phenotype and a strong suppressive capacity to intra-tumoral regulatory T-cells. In particular, the ICOS/ICOSL pathway is required for optimal antitumor responses mediated by anti–CTLA-4 therapy.

References

Kieu-Suong, L., et al. Cancer Res. 2016, 76(16): 1–13.
Fu, T., et al. Cancer Res. 2011, 71(16): 1–10.

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ICOSL:ICOS[Biotinylated] Inhibitor Screening Assay Kit

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