Human EGFRvIII CHO K1 Cell Line (High or Low Expression)

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Catalog #

78145

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Description

Recombinant hEGFRvIII-CHO K1 cell line stably expressing full-length human EGFRvIII receptor (accession number NP_001333870). Surface expression of hEGFRvIII was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of hEGFRvIII expression (High and Low) to mimic different stages of cancer target cells with various EGFRvIII expression levels.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

ERBB cells, HER1 cell line, NISBD2 stable cells , PIG61 cells, epidermal growth factor receptor cell line, EGFR-vIII

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Product Data Gallery

Flow cytometry analysis of cell surface expression of hEGFRvIII in Human EGFRvIII CHO K1 Cell Line (High Expression)

Flow cytometry analysis of cell surface expression of hEGFRvIII in Human EGFRvIII CHO K1 Cell Line (Low Expression).

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cell line, Chinese Hamster Ovary, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains ˃1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Materials Required for Cell Culture

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3B	BPS Bioscience #79529

UniProt #

P00533

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

The epidermal growth factor receptor (EGFR, ErbB-1, HER1) belongs to a family of receptor tyrosine kinases that includes three other closely-related members (HER2, HER3 and HER4). EGFR gene amplification, mutation and re-arrangement are frequently observed in primary glioblastoma multiforme (GBM). The most common EGFR variant in GBM, EGFRvIII, is characterized by a deletion of 267 amino acids in the extracellular domain, leading to a receptor that is unable to bind ligand yet is constitutively active. EGFRvIII enhances the tumorigenic potential of GBM by activating and sustaining mitogenic, anti-apoptotic, and pro-invasive signaling pathways.

Storage/Stability

Cells will arrive upon dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at [email protected] if the cells are not frozen in dry ice upon arrival.

Culture Conditions

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience, #60186): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3B (BPS Bioscience, #79529): F-12K Medium supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 500 μg/ml of Hygromycin B to ensure the recombinant expression is maintained.

Applications

Screen for inhibitors of EGFRvIII signaling for immuno-oncology research and drug discovery.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Kinase

Regulatory Status

Research Use Only

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Human EGFRvIII CHO K1 Cell Line (High or Low Expression)

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