Human CD4 Lentivirus (Hygromycin)

Catalog #

78987

$995 *

Size: 2 x 500 µl

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The Human CD4 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain CD4 (NM_000616.5) driven by an EF1a promoter, and a hygromycin selection marker (Figure 1).

Figure 1. Schematic of the lenti-vector used to generate the Human CD4 Lentivirus.

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Synonyms

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, CD4

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Product Data Gallery

CD4 expression in Jurkat cells transduced with Human CD4 Lentivirus analyzed by flow cytometry.

Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 µl x 2) of lentiviruses at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Materials Required But Not Supplied

Name	Ordering Infromation
Cells of interest
Thaw Medium 2	BPS Bioscience #60184
Lenti-Fuse™ Polybrene Viral Transduction Enhancer	BPS Bioscience #78939
96-well tissue culture-treated assay plates
Flow cytometer

Formulation

The lentiviruses were produced from HEK293T cells. Supplied in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations and produced at higher titers by special request, for an additional fee.  

UniProt #

P01730

Background

CD4 is a cell surface glycoprotein found on partially defined functional T-cell subsets, including helper T cells and T-regulatory cells, peripheral monocytes and other APCs (antigen-presenting cells). The ectodomain of CD4 binds to membrane-proximal domains of MHC (major histocompatibility complex) class II molecules, while its cytoplasmic domains interact with the protein tyrosine kinase p56lck (lck) through a shared cysteine-containing motif. CD4⁺ T cells, or helper T cells, are one type of lymphocyte that helps coordinate the immune response against infection and disease by activating cells of the innate immune system, B-lymphocytes and cytotoxic T cells. CD4⁺ T cells are activated by interaction between the TCR (T- cell receptor) and its cognate peptide presented on MHC II molecules, and CD4 is a critical component of the T cell receptor complex that recognizes antigenic peptides presented by MHC II molecules, increasing its stability. CD4 is a typically used T cell marker that allows to characterize the populations of T cells present.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.

Applications

Study CD4-MHC class II interactions.
Generation of cell pools or stable cell lines expressing CD4 following hygromycin selection.

Shipping Temperature

-80°C

Notes

To generate a CD4 expressing stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of hygromycin (as pre-determined from a killing curve, https://bpsbioscience.com/cell-line-faq), for antibiotic selection of transduced cells, followed by clonal selection. 

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Biosafety
The lentiviruses are produced with a SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and after integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immune Cell Markers

Regulatory Status

Research Use Only

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Human CD4 Lentivirus (Hygromycin)

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