GRK7, GST-tag Recombinant

Catalog #

40108

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human GRK7, also known as GPRK7 (GenBank Accession No. NM_139209), full length, with N-terminal GST tag, MW ~89 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

GPRK7

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

89 kDa

Amino Acids

full length

Specific Activity

2.5 pmol/min/µg

Genbank #

NM_139209

UniProt #

Q8WTQ7

Tag(s)

N-terminal GST-tag

Background

GRK7 is a member of the G protein-coupled receptor kinase subfamily and is a Ser/Thr protein kinase. GRK7 is specifically expressed in the retina and has been shown to phosphorylate cone opsins and initiate their deactivation (1). GRK7 catalyzes rhodopsin phosphorylation in a light-dependent manner. GRK7 co-localizes with GRK1 in human cone outer segments. PKA phosphorylates GRK7 at Ser(23) and Ser(36) in vitro and phosphorylation of GRK7 by PKA reduces the ability of GRK7 to phosphorylate rhodopsin in vitro (2). Since exposure to light causes a decrease in cAMP levels in rod cells, it is proposed that phosphorylation of GRK7 by PKA occurs in the dark.

References

1. Chen, C-K. et al: Characterization of human GRK7 as a potential cone opsin kinase. Molec. Vision 7: 305-313, 2001.
2. Horner, T J. et al: Phosphorylation of GRK1 and GRK7 by cAMP-dependent protein kinase attenuates their enzymatic activities. J Biol Chem. 2005 Aug 5;280(31):28241-50.

Storage/Stability

>6 months at -80°C.

Assay Conditions

5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate GRK7 with 0.25 mg/ml Myelin basic Protein (substrate) and 50 ?M [³³P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40108_Lot110419.pdf

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GRK7, GST-tag Recombinant

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