GRK5, GST-tag Recombinant

Catalog #

40063

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human GRK5, also known as GPRK5 (GenBank Accession No. NM_005308), full length, with N-terminal GST tag, MW ~95 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

GPRK5

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

95 kDa

Amino Acids

full length

Specific Activity

≥33 pmol/min/µg

Genbank #

NM_005308

UniProt #

P34947

Tag(s)

N-terminal GST-tag

Background

GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family (1). It phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. GRK5 plays a role in regulating the motility of polymorphonuclear leukocytes (PMNs). Desensitization of G protein-coupled receptors regulates the number of polymorphonuclear leukocytes (PMNs), as well as their motility and ability to stop upon contact with pathogens or target cells, and this desensitization is mediated by GRK5 (2).

References

1. Haribabu, B. et al: Identification of additional members of human G-protein-coupled receptor kinase multigene family. Proc. Nat. Acad. Sci. 90: 9398-9402, 1993.
2. Fan, J. et al: Toll-like receptor-4 (TLR4) signaling augments chemokine-induced neutrophil migration by modulating cell surface expression of chemokine receptors. Nature Med. 9: 315-321, 2003.

Storage/Stability

>6 months at -80°C.

Assay Conditions

5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate GRK5 with 0.5 mg/ml casein substrate and 50 ?M [³³P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40063_Lot110419.pdf

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