GRK6, GST-tag Recombinant

Catalog #

40066

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human GRK6, also known as GPRK6 or FLJ3213 (GenBank Accession No. BC_017272), full length, with N-terminal GST tag, MW ~93 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

GPRK6; FLJ32135

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

93 kDa

Amino Acids

full length

Specific Activity

11 pmol/min/µg

Genbank #

BC017272

UniProt #

P43250

Tag(s)

N-terminal GST-tag

Background

GRK6 is a Ser/Thr protein kinase and a member of the G protein-coupled receptor kinase subfamily. GRK6 is present in all tissues with strongest expression in placenta and skeletal muscle. GRK6 can phosphorylate proteins such as rhodopsin, ADRB2 and the leukotriene B4 receptor BLT1 (1). GRK6 phosphorylates G protein-coupled dopamine receptors, thereby regulating their activity and mediating desensitization of the receptors. GRK6 knockout mice showed supersensitivity to the locomotor-stimulating effects of psychostimulants, including cocaine and amphetamine (2).

References

1. Gaudreau, R. et al: Threonine 308 within a putative casein kinase 2 site of the cytoplasmic tail of leukotriene B(4) receptor (BLT1) is crucial for ligand-induced, G-protein-coupled receptor-specific kinase 6-mediated desensitization. J. Biol. Chem. 277: 31567-31576, 2002.
2. Gainetdinov, R R. et al: Dopaminergic supersensitivity in G protein-coupled receptor kinase 6-deficient mice. Neuron 38: 291-303, 2003.

Storage/Stability

>6 months at -80°C.

Assay Conditions

5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl₂, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate GRK6 with 0.25 mg/ml PLKtide(substrate) and 50 ?M [³³P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40066__Lot110215.pdf

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GRK6, GST-tag Recombinant

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