Glycophorin A (CD235a) CHO Cell Line

Catalog #

78353

$7,150 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant clonal CHO-K1 stable cell line constitutively expressing full-length human Glycophorin A (GYPA, transcript variant 1, Seq ID: NM_002099.8). The surface expression of Glycophorin A was validated by flow cytometry.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD_antigen: CD235a, GYPA, GPA

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Product Data Gallery

Cell surface expression of Glycophorin A

Product Info

Storage and Usage

Citations

Host Cell Line

CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent

Species

Human

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 3	BPS Bioscience #60186
Growth Medium 3D	BPS Bioscience #79539

UniProt #

P02724

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

Glycophorin A (GYPA, also known as CD235a) is a sialoglycoprotein and a major intrinsic membrane protein on the surface of human erythrocytes. GYPA plays an important role in the prevention of red cell aggregation in the circulatory system. The Glycophorin A gene contains some antigenic alleles of the MNS blood grouping system for which 40 known variants exist. Several of these antigenic variants have implications for pathogen interaction. For example, the Wright b antigen in the helical region of Glycophorin A acts as a receptor for the malaria parasite Plasmodium falciparum. Other variations such as the Mur phenotype causes hemolytic transfusion reaction (HTR) and hemolytic disease in the newborn fetus (HDFN). Glycophorin A is one of the most abundant integral proteins of the red cell membrane, and its genetic sequence varies within a population; therefore, it may also support applications in forensic science.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience #60186):
F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) supplemented with 10% FBS, 1% Penicillin/Streptomycin

Growth Medium 3D (BPS Bioscience #79539):
F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) supplemented with 10% FBS, 1% Penicillin/Streptomycin plus 1 mg/ml Geneticin

Applications

Development and optimization of Glycophorin A-binding antibodies or related peptides
Assay Development

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only