CD235a Positive Cell Isolation Kit

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Catalog #

78896

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Description

The CD235a Positive Cell Isolation Kit is designed to magnetically separate CD235a-expressing cells from a complex immune cell population. This kit is optimized for the isolation of CD235a positive cells from human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, the CD235a-positive cells are immobilized along the side of the tube while undesired CD235a-negative cells remain in suspension and can be easily removed.

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Flow cytometry analysis of CD235+ cells pre- and post-isolation. 1 x 10⁸

Flow cytometry analysis of CD235+ cells pre- and post-isolation. 1 x 10⁹

Product Info

Storage and Usage

Citations

Supplied As

This kit provides enough reagents and materials for isolation from up to 1 x 10⁸ or 1 x 10⁹ PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC samples.

Materials Required But Not Supplied

Peripheral blood mononuclear cells (PBMCs) (BPS Bioscience #79059)
Cell Isolation Magnetic Tube Rack (BPS Bioscience #78571)
Centrifuge
15- or 50-ml tubes
Cell counter

Format

1 x 10⁸ cells

Catalog #	Name	Amount	Storage
	Cell Isolation Magnetic Beads	200 µl	+4°C
	CD235a Cell Isolation Antibody	40 µl	-20°C
78563	5x Cell Isolation Buffer	25 ml	+4°C

1 x 10⁹ cells

Catalog #	Name	Amount	Storage
	Cell Isolation Magnetic Beads	2 ml	+4°C
	CD235a Cell Isolation Antibody	400 µl	-20°C
78563	5x Cell Isolation Buffer	250 ml	+4°C

Background

Erythroid cells are non-nucleated cells responsible for carrying oxygen from the lungs to tissues throughout the body. These cells are of interest in the study of fetal erythroblasts in maternal PBMCs and red blood cell disorders. Depletion of these cells from a mixed sample also allows to study other immune cells. CD235a, also known as glycophorin A, is a marker of erythroid precursors and erythroid cells. In PBMCs derived from healthy individuals, about 0.1-0.2% of the cells are CD235a⁺ cells.

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CD235a Positive Cell Isolation Kit

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