FGFR4 Kinase Assay Kit

Catalog #
78816
$535 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The Fibroblast growth factor receptor 4 (FGFR4) Kinase Assay Kit is designed to measure FGFR4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent. 

Synonyms
Fibroblast growth factor receptor 4, FGFR-4, CD334, JTK2, TKF, FGFR-4
Product Info
Storage and Usage
Citations
Assay Kit Format
Luminescent
Supplied As
The assay kit comes in a convenient 96-well format, with enough purified recombinant FGFR4 kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Materials Required But Not Supplied
  • ADP-Glo® Kinase Assay (Promega #V6930)
  • DTT (Dithiothreitol), 1M, optional
  • Microplate reader capable of reading luminescence
  • Adjustable micropipettor and sterile tips
  • 30°C incubator 
Format
Catalog # Name Amount Storage
40213 FGFR4* 20 µg -80°C
79334 Kinase Assay Buffer 1 (5x) 1.5 ml -20°C
79686 ATP (500 µM) 50 µl -20°C
40217 PTK Substrate (Poly-Glu,Tyr 4:1) (10 mg/ml) 50 µl -20°C
79696 White 96-well plate 1 Room Temp

*The concentration of the protein is lot-specific and will be indicated on the tube

UniProt #
P22455
Background

FGFR4 (also known as CD334) is a transmembrane receptor with tyrosine kinase activity. The extracellular portion of the receptor contains three immunoglobulin-like domains and is responsible for binding fibroblast growth factor 1 (FGF1). It contains a single transmembrane domain followed by the cytosolic kinase domain. FGFR4 may be involved in ovarian cancer as well as adrenocortical carcinoma and is a promising therapeutic target in oncology applications.