The Fibroblast growth factor receptor 4 (FGFR4) Kinase Assay Kit is designed to measure FGFR4 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
The assay kit comes in a convenient 96-well format, with enough purified recombinant FGFR4 kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.
*The concentration of the protein is lot-specific and will be indicated on the tube
UniProt #
P22455
Background
FGFR4 (also known as CD334) is a transmembrane receptor with tyrosine kinase activity. The extracellular portion of the receptor contains three immunoglobulin-like domains and is responsible for binding fibroblast growth factor 1 (FGF1). It contains a single transmembrane domain followed by the cytosolic kinase domain. FGFR4 may be involved in ovarian cancer as well as adrenocortical carcinoma and is a promising therapeutic target in oncology applications.
Storage/Stability
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Applications
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
The final concentration of DMSO in the assay should not exceed 1%.
Warnings
Avoid freeze/thaw cycles
Disclaimers and Limitations
BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.
End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.