FGFR3 (V443L) Assay Kit
The FGFR3 (V443L) Assay Kit is designed to measure FGFR3 (V443L) activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
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Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 0.5 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator
| Catalog Number |
Reagent | Amount | Storage | |
| 100138 | FGFR3 (V443L), His-Tag | 40 µg | -80°C | Avoid multiple freeze/ thaw cycles! |
| 79334 | 5x Kinase assay buffer 1 | 1.5 ml | -20°C | |
| 79686 | ATP (500 µM) | 100 µl | -20°C | |
| 40217 | Protein Tyrosine Kinase Substrate (Poly-Glu, Tyr 4:1)(10mg/ml) |
100 µl | -20°C | |
| 79696 | 96-well plate, white | 1 | Room Temp. | |
1. Cappellen, D., et al. (1999) "Frequent activating mutations of FGFR3 in human bladder and cervix carcinomas." Nature Genetics 23(1): 18.
2. Billerey, C., et al. (2001) "Frequent FGFR3 mutations in papillary non-invasive bladder (pTa) tumors." Amer. J.Pathology 158(6): 1955-1959.
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