FGFR3 (CD333), GST-tag Recombinant

Catalog #

40212

$410 *

Size: 10 µg

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Description

Recombinant human FGFR3 (fibroblast growth factor receptor 3), encompassing amino acids 397-end. This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified and is active.

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Synonyms

ACH, CEK2, JTK4, CD333, HSFGFR3E, FGFR-3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Construct

FGFR3 (GST-397-end)

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM Glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, and 25% glycerol

73 kDa

Amino Acids

397–end

Specific Activity

112 pmol/min/µg

Genbank #

NM_000142

UniProt #

P22607

Tag(s)

N-terminal GST-tag

Background

Fibroblast growth factor receptor 3 (FGFR3) is part of a family of fibroblast growth factor receptors that share similar structures and functions. FGFR3 plays a role in several important cellular processes, including regulation of cell growth and division, determination of cell fate, formation of blood vessels, wound healing and embryo development (1). FGFR3 is involved in the development and maintenance of bone and brain tissue. Mutations in FGFR3 have been implicated in causing bladder cancer, cancer of white blood cells (multiple myeloma) and cervical cancer (2).

References

1. Chen, L. and Deng, C.X. Roles of FGF signaling in skeletal development and human genetic diseases. Front Biosci. 2005; 1(10):1961-1976.
2. Mhawech-Fauceglia, P. et al. 2006. FGFR3 and p53 protein expressions in patients with pTa and pT1 urothelial bladder cancer. Eur. J. Surg. Oncol. 2006; 32(2):231-237

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

FGFR3 activity was measured by using the Poly(Glut4Tyr1) synthetic peptide, diluted in 25 mM Tris-HCl (pH 7.5) to a working concentration of 1 mg/ml, in the ADP Glo™ Kinase Assay kit (Promega #V9101). Reaction was initiated by mixing increasing amounts of FGFR3 with 25 μM ATP in 40 mM Tris-HCl, pH 7.4, 20 mM MgCl₂, 0.1 mg/ml BSA prepared with 50 μM DTT, 12.5 mM MnCl₂ and substrate at a final concentration of 200 µg/ml.

After a 40-minute incubation at room temperature, the reaction was terminated by addition of ADP-Glo™ Reagent, followed by a subsequent 40-minute incubation at room temperature. Kinase Detection Reagent was added, and the reaction was incubated for another 30 minutes at ambient temperature. Detection of luminescence was measured using the Luminescence Module Protocol on GloMax®-Multi Microplate Multimode Reader. The Specific Activity was calculated as follows: (Corrected activity, RLU) / [(Specific activity from ADP in RLU/pmol) * (Reaction time in min) *(Enzyme amount in μg or mg)]. Corrected RLU was calculated by subtracting the blank value from all the values. The blank was determined from a “no enzyme” sample by replacing the enzyme solution with an equal volume of Kinase Dilution Buffer X (1x).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Tyrosine Kinase

Regulatory Status

Research Use Only

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Datasheets/Protocols

40212 Lot 240124

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FGFR3 (CD333), GST-tag Recombinant

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