FcRL5/Firefly Luciferase RPMI 8226 Cell Line

Catalog #

78378

$10,340 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant RPMI 8226 cells constitutively expressing firefly (Photinus pyralis) luciferase (BPS Bioscience #79834) have also been engineered to constitutively express the full length human FcRL5 (Fc Receptor Like 5, also known as CD307, ref. seq. NM_031281).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Synonyms

Fc receptor-like protein 5, FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, FcRH5, Immune receptor translocation-associated protein 2, CD307e, IRTA2, UNQ503, PRO820

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Product Data Gallery

Analysis of FcRL5 expression in FcRL5/Firefly Luciferase RPMI 8226 cells

Luciferase activity in the FcRL5/Luciferase RPMI 8226 Cell Line

Product Info

Storage and Usage

Citations

Host Cell Line

RPMI 8226, human B cells isolated from a plasmacytoma/myeloma patient, suspension

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of cell freezing medium (BPS Bioscience, #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 10	BPS Bioscience #79704
Growth Medium 10B	BPS Bioscience #78381

UniProt #

Q96RD9

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

FcRL5 is a single-pass type 1 membrane protein which contains 8 immunoglobulin-like C2-type domains. FcRL5 may be involved in B cell development and lymphomagenesis. Moreover, it is enriched in malignant plasma cells of patients diagnosed with multiple myeloma, and is an attractive target for antibody-drug conjugates (ADC) and anti-FcRL5 CAR T cells. RPMI 8226 cells are human B cells isolated from a plasmacytoma/myeloma patient.

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 10 (BPS Bioscience, #79704): RPMI 1640 medium supplemented with 10% FBS, 1mM Sodium pyruvate, 1% Non-essential amino acids, 1% Penicillin/streptomycin.

Growth Medium 10B (BPS Bioscience, #78381): RPMI 1640 medium supplemented with 10% FBS, 1mM Sodium pyruvate, 1% Non-essential amino acids, 1% Penicillin/streptomycin plus 400 µg/ml Geneticin and 0.125 µg/ml puromycin.

Applications

Use as target cells for anti-FcRL5 CAR T cells, anti-FcRL5 ADC, and bi-specific antibodies.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only