FcRL5 Lentivirus (Macaca fascicularis/Cynomolgus)

Catalog #

78781

$850 *

Size: 500 µl x 2

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Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The FcRL5 (Fc receptor-like protein 5) Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce cells with Macaca fascicularis (also known as crab-eating macaque or cynomolgus monkey) FcRL5 (XP_005541423.1) driven by an EF1a promoter. The lentiviruses also transduce a puromycin selection gene (Figure 1).

Figure 1: Schematic of the lenti-vector used to generate the cynomolgus FcRL5 Lentivirus.

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Product Data Gallery

Expression of FcRL5 in CHO-K1 cells using cynomolgus FcRL5 lentivirus.

Product Info

Storage and Usage

Citations

Supplied As

Two vials (500 µl x 2) of lentivirus at a titer ≥10⁷ TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.

Formulation

The lentivirus particles were produced in HEK293T cells in medium containing 90% DMEM + 10% FBS. Virus particles can be packaged in custom formulations by special request, for an additional fee.

Background

FcRL5 (Fc receptor-like protein 5, also known as IRTA2) is a single-pass transmembrane protein of the immunoglobulin superfamily (IgSF) containing 8 immunoglobulin-like C2- type domains in its extracellular portion, and a short cytoplasmic tail. FcRL5 is expressed in the B cell lineage and may be involved in B cell development and lymphomagenesis. Unlike CD20, CD19 and CD22, FcRL5 is still expressed in plasma cells. It is enriched in malignant plasma cells of patients diagnosed with multiple myeloma (MM). FcRL5 has been used as a target in antibody-drug conjugates (ADC) and antiFcRL5 CAR-T cells, with data so far indicating they are effective in the treatment of MM.

Storage/Stability

Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. Titers can drop significantly with each freeze/thaw cycle.

Applications

Expression of FcRL5 in cells of interest.
Generate stable cell lines expressing cynomolgus FcRL5 (puromycin resistant).

Shipping Temperature

-80°C

Notes

To generate a FcRL5 stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of puromycin (as pre-determined from a killing curve), for an antibiotic selection of transduced cells. Visit: https://bpsbioscience.com/cell-line-faq for guidelines on performing a kill curve.

Biosafety
None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.

Troubleshooting Guide
Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For further questions, email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR T-Cell Therapy

Regulatory Status

Research Use Only

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FcRL5 Lentivirus (Macaca fascicularis/Cynomolgus)

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