eGFP/Firefly Luciferase MIA PaCa-2 Cell Line

Catalog #

78766

$3,250 *

Size: 2 vials

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Recombinant MIA PaCa-2 cells expressing firefly luciferase and enhanced GFP (eGFP) driven by an EF1a promoter, generated by transduction with Firefly Luciferase-eGFP lentivirus (BPS Bioscience #78741).

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

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Product Data Gallery

eGFP expression and Luciferase activity in GFP/Firefly Luciferase MIA PaCa-2 cells.

Product Info

Storage and Usage

Citations

Host Cell Line

MIA PaCa-2, human pancreatic carcinoma cell line, adherent.

Supplied As

Each vial contains 2 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Materials Required But Not Supplied

Name	Ordering Information
Thaw Medium 17	BPS Bioscience #78767
Growth Medium 17A	BPS Bioscience #78768

Mycoplasma Testing

The cell line has been screened to confirm the absence of Mycoplasma species.

Background

MIA PaCa-2 is a human epithelial cell line derived from the pancreatic cancer tissue of a 65-year-old male. It is commonly used as a tumor model in pancreatic cancer research and therapy development. The genotyping of MIA PaCa-2 confirmed the presence of a homozygous missense mutation (G12C) in KRAS (K-Ras), a homozygous deletion encompassing exons 1, 2 and 3 of the CDKN2A/p16INK4A (cyclin-dependent kinase inhibitor 2A) gene and a homozygous missense mutation (R248W) in exon 7 of TP53 (tumor protein 53, or p53).

Storage/Stability

Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Growth Media

For best results, the use of validated and optimized media from BPS Bioscience is highly recommended. Other preparations or formulations of media may result in suboptimal performance.

Media Required for Cell Culture
Thaw Medium 17 (BPS Bioscience #78767): DMEM supplemented with 10% FBS, 2.5% horse serum, 1% Penicillin/Streptomycin.

Growth Medium 17A (BPS Bioscience #78768): DMEM supplemented with 10% FBS, 2.5% horse serum, 1% Penicillin/Streptomycin plus 1 µg/ml Puromycin.

Media Required for Functional Cellular Assay
Thaw Medium 17 (BPS Bioscience #78767): DMEM supplemented with 10% FBS, 2.5% horse serum,1% Penicillin/Streptomycin.

Applications

Use as target cells in CAR-T or NK co-culture killing assays.
In vitro and in vivo bioluminescence imaging (BLI).

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid freeze/thaw cycles

Scientific Category

CAR T-Cell Therapy

Regulatory Status

Research Use Only

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eGFP/Firefly Luciferase MIA PaCa-2 Cell Line

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