DNMT3A/DNMT3L Complex, His-tag, GST-tag Recombinant

Catalog #

51106

$475 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Complex of active human DNA methyl-transferase 3A (DNMT3A), GenBank Accession No. NM_175629, a.a 623-912(end) with N-terminal His-tag, MW=34 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal GST-tag, MW = 52 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.

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Synonyms

DNMT-3A, DNMT-3L

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Product Data Gallery

10% SDS-PAGE Coomassie Staining

Specific Activity

DNMT3A inhibition curve using a chemiluminescent assay (#52033). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.

86 kDa (complex)

Amino Acids

DNMT3A: 623-912 (end), DNMT3AL: 160-387(end)

Specific Activity

≥0.58 pmol/min/µg.

Genbank #

NM_175629(DNMT3A), NM_013369 (DNMT3L)

UniProt #

Q9Y6K1 (DNMT3A), Q9UJW3 (DNMT3L)

Tag(s)

N-terminal His, N-terminal GST-tag

Storage/Stability

>6 months at –80°C.

Assay Conditions

Assayed using BPS Bioscience DNMT3A Assay Kit, Cat. #52033. Add 50 µl reaction mix containing DNMT3A/DNMT3L to wells coated with DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only