DNMT3B/DNMT3L Complex, GST-tag, His-tag Recombinant

Catalog #

51109

$475 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Complex of human DNA methyl-transferase 3B (DNMT3B), GenBank Accession No. NM_006892, a.a. 564-853(end) with N-terminal GST-tag, MW=59 kDa, and DNMT3L (GenBank Accession No. NM_013369), a.a. 160-387(end), with an N-terminal His-tag, MW = 27 kDa, co-expressed in a baculovirus-infected Sf9 cell expression system.

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Synonyms

DNMT-3B, DNMT-3L

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Product Data Gallery

DNMT3B/DNMT3L Activity

10% SDS-PAGE Coomassie Staining

DNMT3B inhibition curve using a chemiluminescent assay (#52034). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, and 20% glycerol

86 kDa (complex)

Amino Acids

564–853(end), DNMT3b; 160–387(end), DNMT3L

Specific Activity

≥0.01 pmol/min/µg.

Genbank #

NM_006892(DNMT3B), NM_013369 (DNMT3L)

UniProt #

Q9UBC3 (DNMT3B), Q9UJW3 (DNMT3L)

Tag(s)

N-terminal GST (DNMT3B) N-terminal His-tag (DNMT3L)

Storage/Stability

>6 months at –80°C.

Assay Conditions

Assayed using BPS Bioscience DNMT3B Assay Kit, Cat. #52034. Add 50 µl reaction mix containing DNMT3B/DNMT3L to wells coated with DNMT substrate. Incubate 2 hr at 37°C. Add antibody against 5-methylcytosine, then HRP-labeled secondary antibody followed by chemiluminescent detection.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only