DNMT1, GST-tag Recombinant

Catalog #

51101

$485 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human DNMT1 (DNA methyltransferase 1, isoform b ), full-length, encompassing amino acids 2-1632(end). This construct contains an N-terminal GST-tag and includes a thrombin cleavage site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

This product has been cited 6 times.

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Synonyms

TRDMT1, DNA, ADCADN, AIM, CXXC9, DNMT, HSN1E, MCMT, m.HsaI, DNA (cytosine-5-)-methyltransferase 1, DNMT-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

DNMT1 Activity

DNMT1 inhibition curve using a chemiluminescent assay (#52050L). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations6

Species

Human

Construct

DNMT1 (GST-2-1632(end))

Host Species/Expression System

Sf9

Purity

≥78%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT

211 kDa

Amino Acids

2-1632(end)

Genbank #

NM_001130823

UniProt #

P26358-2

Tag(s)

N-terminal GST-tag

References

1. Tan, H.H. and Porter, A.G., Biochem. Biophys. Res. Commun. 382 (2):462-467 (2009).
2. Fan, H., et al., World J. Gastroenterol. 15 (16):2020-2026 (2009).

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was performed according to the protocol in DNMT1 Chemiluminescent Assay Kit (BPS Bioscience #52050L) with DNMT1 titrated at various concentrations

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Methyltransferase

Regulatory Status

Research Use Only