DNMT1 Chemiluminescent Assay Kit
The DNMT1 Assay Kit is designed to measure DNMT1 activity for screening and profiling applications. The key to the DNM1 Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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This product has been cited 4 times.
TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween20)
Luminometer or microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
0.5M DT
Catalog Number |
Component |
Amount |
Storage |
|
51101 | DNMT1 | 2 x 10 μg | -80°C | Avoid freeze/ thaw cycles! |
52120 | 400 μM S-adenosylmethionine | 2 x 250 μl | -80°C | |
Anti-5-methylcytosine antibody | 25 µl | -80°C | ||
52130H | Secondary HRP-labeled antibody 1 | 10 µl | -80°C | |
52200 |
4x DNMT assay buffer 1* *Add 260 μl of 0.5 M DTT before use. |
5 ml |
-20°C |
|
52100 | Blocking buffer 4 | 50 ml | +4°C | |
HRP chemiluminescent substrate A (translucent bottle) |
6 ml | +4°C | ||
HRP chemiluminescent substrate B (brown bottle) |
6 ml |
+4°C |
||
Plate precoated with DNMT1 substrate | 1 | +4°C |
Svedruzic, Z.M., Curr Med Chem. 2008;15(1):92-106.