CSK Kinase Assay Kit
The C-terminal Src kinase (CSK) Kinase Assay Kit is designed to measure CSK kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
- ADP-Glo® Kinase Assay (Promega #V6930)
- DTT (Dithiothreitol), 1M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40410 | CSK* | 5 µg | -80°C |
| 79334 | Kinase Buffer 1 (5x) | 1.5 ml | -20°C |
| 79686 | ATP (500 µM) | 50 µl | -20°C |
| 40217 | PTK Substrate (poly-Glu,Tyr 4:1) (10 mg/ml) | 50 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube
C-terminal Src kinase (CSK) is a non-receptor tyrosine kinase encoded by the CSK gene and plays a prominent role in immune cells. Upon activation of T cell receptors or B cell receptors, CSK is recruited to the plasma membrane from the cytosol by an adapter protein and phosphorylates tyrosine residues present in the C-terminal end of Src-family kinases to downregulate their activity. Targets of CSK include Scr, Fyn, Yes and lymphocyte-specific protein tyrosine kinase (LCK). Thus, CSK is involved in a wide variety of signal transduction cascades.
Powered by Bioz
San Diego, CA 92121
858-202-1401
Products are for Research Use Only
COMPANY
Copyright © 2024
BPS Bioscience, Inc.
All rights reserved.
Diagrams on this website are created with BioRender.com.