Claudin-6, FLAG-Tag Recombinant

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Catalog #

101599

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Description

Recombinant human claudin-6, full length encompassing amino acids 1-220 (end). This construct contains a C-terminal FLAG-tag. This protein was affinity purified.

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Synonyms

Skullin, CLDN6, UNQ757/PRO1488

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Claudin-6: Anti-Claudin-6 Antibody Binding Assay

Product Info

Storage and Usage

Citations

Species

Human

Construct

Claudin-6 (1-220(end)-FLAG)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution.

Formulation

50 mM HEPES, pH 8.0, 300 mM NaCl, 10% Glycerol, 0.1% DDM (dodecyl-β-D-maltoside), 0.01% CHS (cholesteryl hemisuccinate), and 140 µg/ml FLAG peptide

23 kDa

Amino Acids

1-220(end)

Genbank #

NM_021195.5

UniProt #

P56747

Background

Claudins are integral membrane proteins and major components of tight junction strands which serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Claudin-6 is one of the 27 members of the claudin family. Claudin-6 is widely expressed in various tumors but rarely expressed in healthy adult tissues. This cancer specific expression of claudin-6 makes it a promising target as an antigen for cancer immunotherapy. Several therapeutics that target claudin-6 are currently in development or in clinical trials including bispecific antibodies, monoclonal antibodies, and CAR-T cell therapy products.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Binding to Anti-Claudin-6 antibody (Santa Cruz Biotechnology #sc-393671) was performed using ELISA. The Claudin-6 protein was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) containing DDM/CHS detergent (DDM 0.05%, CHS 0.01%), and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) with DDM/CHS for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-Claudin-6 antibody serial dilutions in Blocking Buffer 2 with DDM/CHS detergent was added and incubated for 45 minutes at room temperature. After 3 washes, the plate was incubated with Anti-Mouse IgG H&L (HRP) (Abcam #ab7061), washed 3 times, and incubated with Colorimetric HRP substrate (BPS Bioscience #79651). The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for binding assays and SDS-PAGE.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Cancer Therapy Target

Regulatory Status

Research Use Only

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Datasheets/Protocols

101599 Lot 230921

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Claudin-6, FLAG-Tag Recombinant

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