Chemi-Verse™ Aurora Kinase A Assay Kit
The Chemi-Verse™ Aurora Kinase A Assay Kit is a luminescence assay designed to measure Aurora Kinase A activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase A, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
- ADP-Glo™ Kinase Assay (Promega #V6930)
- DTT (Dithiothreitol), 1M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40004 | Aurora Kinase A, His-Tag (Sf9-derived)* | 5 µg | -80°C |
| 79334 | 5x Kinase Buffer 1 | 1.5 ml | 20°C |
| 79686 | 500 µM ATP | 50 µl | 20°C |
| Kemptide (5 mg/ml) | 100 µl | 20°C | |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
Aurora Kinase A, also known as serine/threonine-protein kinase 6, is part of the Aurora subfamily. Aurora Kinase A is a CPC (chromosomal passenger complex) protein. It plays a role in microtubule organization during mitosis e meiosis. It recruits several proteins required for mitotic spindle formation, such as kinesin 5 and γ-tubulin, and its interaction with the kinetochore. It has a pattern of expression and subcellular localization that varies with the cell cycle stage, in agreement with its role as cell cycle regulator. It has oncogenic activity and is overexpressed in cancer tissues like breast cancer and prostate cancer. Aurora Kinase A dysfunction contributes to the development of cancer in multiple ways, which include activating the NF-κB pathway, increasing anti-apoptotic factors (Bcl-2, B-cell lymphoma 2, and MCL-1) and increase of EMT (epithelial to mesenchymal transition) related transcription factors. The use of inhibitors for Aurora Kinase A can support recovery in cases of cancer where drug resistance has occurred. Further studies will elucidate the exact mode of action of Aurora Kinase A in cancer development and progression and open new therapeutic avenues.
Tang A., et al., 2017 Oncotarget 8(14):23937-23954.
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