Chemi-Verse™ Kinase Aurora C Assay Kit
The Chemi-Verse™ Aurora Kinase C Assay Kit is a luminescence assay designed to measure Aurora Kinase C activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified recombinant Aurora Kinase C, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.
- ADP-Glo™ Kinase Assay (Promega #V6930)
- DTT (Dithiothreitol), 1M, optional
- Microplate reader capable of reading luminescence
- Adjustable micropipettor and sterile tips
- 30°C incubator
| Catalog # | Name | Amount | Storage |
| 40178 | Aurora Kinase C, GST-Tag Recombinant * | 5 µg | -80°C |
| 79334 | 5x Kinase Buffer 1 | 1.5 ml | -20°C |
| 79686 | 500 µM ATP | 50 µl | -20°C |
| 78514 | Myelin basic protein (MBP), 5 mg/ml | 50 µl | -20°C |
| 79696 | White 96-well plate | 1 | Room Temp |
*The concentration of the protein is lot-specific and will be indicated on the tube.
Aurora Kinase C, also known as serine/threonine-protein kinase 13, is part of the Aurora subfamily. Aurora Kinase C is a CPC (chromosomal passenger complex) protein and forms a complex with Aurora Kinase B and centromere proteins. It plays a role in microtubule organization during mitosis and meiosis, mostly in meiosis. It has a pattern of expression and subcellular localization that varies with the cell cycle stage, in agreement with its role in cell cycle regulation. It has oncogenic activity and is overexpressed in cancer cell lines, by performing similar functions to Aurora Kinase B and other still unknown mechanisms. Mutations that inactivate this protein result in male infertility, being the main cause of infertility in North African men. The use of inhibitors can increase the effectiveness of chemotherapy and has been shown to stop the progress and growth of several cancers. Further studies will elucidate the exact mode of action of Aurora Kinase C in cancer development and progression and open new therapeutic avenues.
Tang A., et al., 2017 Oncotarget 8(14):23937-23954.
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