CDK7/CyclinH1/MNAT1, His-tags Recombinant
Complex of CDK7 (cyclin dependent kinase 7), Cyclin H1 and MNAT (MNAT1 component of CDK activating kinase).
Recombinant human CDK7, full length encompassing amino acids 2-346 (end), contains an N-terminal His-tag (6xHis). Recombinant human Cyclin H, full length encompassing amino acids 1-323 (end), contains a C-terminal His-tag (6xHis). Recombinant human MNAT, full length encompassing amino acids 2-309 (end), contains an N-terminal His-tag (6xHis). The three proteins were co-expressed in Sf9 insect cells and spontaneously formed a complex, which was affinity purified.
CDK7: CAK, CDK-activating kinase 1, CAK1, HCAK, MO15, STK1, cell division protein kinase 7, CDKN7, 39 kDa protein kinase, p39MO15
Cyclin H1: CCNH, CAK, p34, p37, CDK-7
≥90%
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and variable imidazole
CDK7 gene is a member of the cyclin-dependent protein kinase family that is important regulators of cell cycle progression (1). CDK7 forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). CDK7 is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. CDK7 is thought to serve as a direct link between the regulation of transcription and the cell cycle (2).
1. Fisher, R. P.;A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase. Cell 78: 713-724, 1994.
2. Larochelle, S. Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells. Molec. Cell 25: 839-850, 2007.
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