Search results for: 'histone'

Citations   This page contains a list of scientific journal articles that have cited BPS products and services since 2013. We would like to congratulate all our loyal customers on their contributions to the scientific community. Our customers are truly amazing and dedicated scientists and this is evidenced by the outstanding research they are doing.  Search By Product Name or Catalog Number Name Title 10X HAT Assay Buffer (50095...

Acetyltransferase Screening & Profiling The transfer of acetyl groups to lysine residues of histone proteins by acetyltransferase enzymes is critical to epigenetic control of genes in cell maintenance and survival. Histone acetyltransferases (HATs) are involved in disease processes as well and have garnered attention as targets for therapy across a number of pathological areas including cancer, neurodegenerative disorders, and inflammatory diseases.  ...

PRMT5/MEP50 Methylation Comparing Methylation Patterns of Recombinant PRMTs:  Abnormal PRMT5/MEP50 Methylation PRMTs – Roles & Mechanisms Protein arginine methyltransferase 5 ( PRMT5 ) is a critical regulatory protein linked to genome organization, cell cycle regulation, and stem cell differentiation 1 . It forms an active complex with MEP50 and transfers methyl groups from S-adenosylmethionine (SAM) to histone proteins, transcription factors, and other regula...

Methyltransferase Screening & Profiling Methyltransferases are enzymes that transfer a methyl group from S-adenosylmethionine (SAM) to substrate molecules, such as DNA, RNA, and proteins. In particular, methylation of lysines or arginines in histone tails provides epigenetic regulation of gene expression, in which methyltransferases serve as the “writers” of the methylation code on histones. The degree of methylation and the location of methylation i...

Bromodomain Screening & Profiling The bromodomain (BRD) family members contain an evolutionarily conserved protein fold that specifically binds acetylated lysine residues. Many bromodomain-containing proteins, such as Type A histone acetyltransferases, are chromatin remodeling enzymes. Other bromodomain-containing proteins act as transcription factors to regulate gene activity. These acetyl-lysine ‘readers’ of the histone code are important regulators of develo...

Demethylase Screening & Profiling Histone demethylases are epigenetic regulators of chromatin structure and gene expression. Due to their deregulation or overexpression in cancer, and in cardiovascular and neurodegenerative diseases, they are important therapeutic targets. Additionally, DNA and RNA demethylation are also linked to tumorigenesis and are considered important targets for cancer therapy as well. We offer an extensive selection...

Epigenetics Modulation of Gene Expression Without Changing the Genome The field of epigenetics refers to the control or modulation of gene expression that produces a specific phenotype or trait without directly changing DNA sequences. Epigenetic control can occur by modifying DNA, RNA, or by modulating the proteins that bind to DNA, particularly histone proteins. Epigenetic modifications start early in embryonic development and continue as organisms age and are strongl...

HDAC/Sirtuin Screening & Profiling Histone deacetylases (HDACs) are negative regulators of gene transcription due to their ability to tightly compact heterochromatin by removing acetyl groups from histone tails. Thus, they are considered important targets for anti-cancer therapeutics due to their involvement in cell cycle regulation, cell proliferation, and apoptosis, and overall gene expression. Similarly, the Sirtuin family of deacetylases are targets ac...

Screening and Profiling Services Evaluate lead compounds with our biochemical and cell-based screening services to identify specific inhibitors or activators. Save time by letting us screen your small molecules or biologics of interest against our unique assay panels or determine IC 50 values. With our portfolio of > 600 assays available as a service, we’re here to help advance your drug discovery program. Our biochemical-based inhibitor...

PARP/PARPTrap™ Screening & Profiling PARP (Poly ADP-Ribose Polymerase) proteins are a large family of 17 members that catalyze the ADP-ribosylation of proteins and DNA. PARPs are part of a network of 150 proteins involved in the DNA damage response, which constantly scan and repair DNA to maintain genome integrity. The PARP proteins are involved in a wide range of biological functions: DNA repair, chromatin remodeling, mitotic spindle assembly, regulati...

Support Products & Consumables  Off-the-Shelf Solutions and Kits for Cell and Protein Analysis In scientific experimentation, even basic components such as buffers and reagents can have a significant influence on the integrity of experimental outcomes. Accurate preparation and proper selection of buffers are crucial for achieving consistent and reliable results. For instance, in processes like cell lysis, the choice of the appropriate buffer can ...

PARP Assays Poly(ADP-ribose) polymerase (PARP) proteins play a crucial role in DNA repair mechanisms within cells. These enzymes are involved in the repair of single-strand DNA breaks through the base excision repair pathway. Inhibition of PARP proteins has emerged as a promising strategy in cancer therapy, particularly in tumors with defective DNA repair mechanisms, such as those with BRCA mutations. When PARP is inhibited, it prevents the repair of single-strand breaks, leading to th...

Methyl-lysine Reader Screening & Profiling Methyl-lysine reader proteins specifically recognize methylated lysine on histone tails and help to convey this epigenetic modification by recruiting activating or repressive factors regulating downstream processes such as cell cycle regulation, stress responses, development processes, or oncogenesis. Their involvement in cancer makes them attractive therapeutic targets. We offer a selection of scr...

NAMPT: Metabolism, Cancer, and Drug Discovery NAMPT & Metabolism The unique metabolic requirements of rapidly reproducing cells are classical targets for cancer treatment.  For example, rapid growth creates a high demand for ATP used in the biosynthesis of DNA and proteins.  The enzymatic cofactors  NAD +  and NADH contribute oxidizing and reducing agents required to generate ATP and regulate the resulting oxidative stress.  This need for high levels of A...

PRMT5 & Cancer   Protein arginine methyltransferase 5 ( PRMT5 ) is a critical regulatory protein linked to genome organization, cell cycle regulation, and stem cell differentiation 1 . It forms an active complex with MEP50 and transfers methyl groups from S-adenosylmethoinine (SAM) to histone proteins, transcription factors, and other regulatory proteins. These methylations form mono and symmetrically di-methylated arginine residues. Dysregulation of PRMT5 activity is assoc...

Setting a Trap for PARP1 and PARP2 Summary PARP1 and PARP2 operate in DNA damage response (DDR) by sensing single breaks in DNA and by recruiting the repair machinery through poly-ADP ribosylation of histones and other proteins. PARP inhibitors are cytotoxic to cancer cells deficient in repair pathways that are complementary to PARP pathways, and it has been shown that some of these inhibitors act by trapping PARP1/2 onto the DNA, preventing repair and leading to cell death. Thus, DNA tra...

Cellular PARylation: From Inquiry to Insight in Drug Discovery Summary Inhibitors of poly (ADP-ribose) polymerases (PARPs) have been game-changing in cancer therapy, demonstrating the potential efficacy of synthetic lethal drugs targeting complementary pathways within the DNA damage response network. However, the evaluation of poly-ADP-ribosylation in intact cells is difficult, slowing the development of candidate drugs. BPS Bioscience’s LysA™ Universal PARylation Assay Kit quantifies...