BRDT (BD1+BD2) TR-FRET Assay Kit
The BRDT (BD1 + BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRDT (BD1 + BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRDT, ligand, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.
Need us to run inhibitor screens or profile your compounds against BRDT (BD1+BD2)? Check out our Bromodomain Screening Services.
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips
| Catalog Number |
Component | Amount | Storage | |
| 31061 | BRDT (BD1+BD2) | 10 µg | -80°C | Avoid freeze/ thaw cycles! |
| 33000 | BET Bromodomain Ligand | 50 µl | -80°C | |
| Non-acetylated Ligand 1 | 15 µl | -80°C | ||
| Tb donor | 2 x 10 µl | -20°C | ||
| Dye-labeled acceptor | 2 x 10 µl | -20°C | ||
| 33012 | 3x BRD TR-FRET Assay Buffer 1 | 4 ml | -20°C | |
| White, Nonbinding, low volume, microtiter plate | 1 | Room Temp. | ||
Filippakopoulos, P., et al., Cell 2012; 149:214.
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