BRDT (BD1), His-tag Recombinant

Catalog #

31101

$305 *

Size: 100 µg

Qty

Bulk/Custom

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Description

Human Bromodomain testis-specific protein, also known as BRDT, BRD6, and CT9, GenBank Accession No. NM_207189, a.a. 22 – 138 corresponding to bromodomain 1 with N-terminal His-tag, MW = 14.9 kDa, expressed in an E. coli expression system.

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Synonyms

Bromodomain testis-specific protein, BRDT, BRD6, and CT9

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

His BRDT-BD1

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, and 10% glycerol

14.9 kDa

Amino Acids

22-138

Genbank #

NM_207189

UniProt #

Q58F21

Tag(s)

N-terminal His-tag

References

1. Berkovits, B.D., et al., Dev. Biol. 2011 Dec 15; 360(2): 358-368.
2. Shang, E., et al., Development. 2007 Oct; 134(19): 3507-3515.

Storage/Stability

>6 months at –80°C.

Assay Conditions

BRDT-BD1 (400 nM) is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) as indicated in BRD assay buffer (BPS #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® Ni2+ acceptor beads (Perkin Elmer) are added, followed by Alphascreen^® Streptavidin-conjugated donor beads (Perkin Elmer). After 1 hour incubation at RT, Alphacounts are measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only