BRD3 (BD1), His-tag Recombinant

Catalog #

31030

$305 *

Size: 100 µg

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Bulk/Custom

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Description

Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29-145 corresponding to bromodomain 1 with N-terminal His-tag, MW = 14.9 kDa, expressed in an E. coli expression system.

This product has been cited 1 time.

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Synonyms

Bromodomain containing 3, BRD3, RING3L, BRD-3

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BrD3-BD1

Product Info

Storage and Usage

Citations1

Species

Human

Host Species/Expression System

E. coli

Purity

≥88%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, and 10% glycerol

14.9 kDa

Amino Acids

29-145

Genbank #

NM_007371

UniProt #

Q15059

Tag(s)

N-terminal His-tag

References

1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.
2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168.

Application References:
1. Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion (2021)

Storage/Stability

>6 months at –80°C.

Assay Conditions

BRD3 is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) in BRD assay buffer (BPS #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® Nickel Chelate acceptor beads (PerkinElmer) are added, followed by Alphascreen^® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour minute incubation at RT, Alphacounts are measured.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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Datasheets/Protocols

31030_Lot120207G.pdf

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BRD3 (BD1), His-tag Recombinant

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