BRD3 (BD1+BD2), GST-tag Recombinant

Catalog #

31035

$305 *

Size: 100 µg

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant human BRD3 (Bromodomain containing 3, also known as RING3L), encompassing amino acids 29-417 corresponding to bromodomains 1 and 2. This construct contains an N-terminal GST-tag. A short peptide linker placed between GST and the protein includes a PreScission Protease Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.

This product has been cited 2 times.

●

Synonyms

Bromodomain containing 3, BRD3, RING3L, BRD-3

●

Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BRD3 (BD1+BD2) Activity

BRD3 (BD1+BD2) inhibition curve using a TR-FRET assay (#32615). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Construct

BRD3 (BD1+BD2) (GST-29-417)

Host Species/Expression System

E. coli

Purity

77%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

70 kDa

Amino Acids

29-417

Genbank #

NM_007371

UniProt #

Q15059

Tag(s)

N-terminal GST-tag

References

Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

Assay was done according to BRD3 (BD1+BD2) TR-FRET Assay Kit (BPS Bioscience #32615) with various amounts of BRD3 (BD1+BD2), GST-tag.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

Shipping Temperature

dry ice

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

●

Datasheets/Protocols

31035 Lot 220127-1

Datasheets provide example lot-specific information. Contact us to request information on currently available lots.

● General Protocols

● FAQs

● Contact Technical Service

●

BRD3 (BD1+BD2), GST-tag Recombinant

PRODUCTS

SERVICES

RESOURCES

COMPANY